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. 2022 Jul 4;13:907145. doi: 10.3389/fgene.2022.907145

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Copyright © 2022 Zhang, Cai, Lu, Xu, Qu, Zhao and Ding.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Validation of the mRNA and protein expression of RFRGs. (A) Score of the 14 RFRGs. The aforementioned heatmap shows the total scores of each gene, and the heatmap below shows the exact scores of seven analyses. (B,C) Degree of renal injury was measured by H&E staining and relative mRNA levels of KIM1 of kidney sections from the cisplatin-induced AKI model at different time points. (D,E) Relative mRNA level and representative immunoblot analysis of the top six RFRGs in the cisplatin-induced AKI model at different time points. (F,G) Degree of renal injury was measured by H&E staining and relative mRNA levels of KIM1 of kidney sections from the CLP-induced AKI model at different time points. (H,I) Relative mRNA level and representative immunoblot analysis of the top six RFRGs in the CLP-induced AKI model at different time points. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 vs. control group.