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. 2022 Jul 4;10:868029. doi: 10.3389/fcell.2022.868029

FIGURE 4.

FIGURE 4

Clarification reveals Schlemm’s canal structure and collector channel relationships. (A) 112-µm thick confocal stack 3D projection of merged channels of CD31 (red) and Col type 1 (green). Clarification permits the appreciation of complex connections of the trabecular meshwork (TM) and Schlemm’s canal (SC) inner wall endothelium (SCE). SC inner wall valve-like structures (SIV) arise from SCE. The SIV attach to the SC external wall area (EXA) at collector channel ostia (CCO) that lead to collector channels (CC). (B,C) Subset stack of (B) 48 µm and (C) 18 µm. CCOs at the external wall lead to three CC traversing the sclera that join a circumferentially oriented intrascleral vessel. (A–D) SIV have a lumen apparent as voids in central sections of serial confocal image stacks (D) stack size is 42 µm. The SIV walls are labeled with CD 31 and Col type 1-like SC inner wall and form a funnel (F) developing into a cylinder attached to the SC external wall confirmed with serial confocal stack images #27L215.