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. 2022 Jul 4;10:868029. doi: 10.3389/fcell.2022.868029

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Copyright © 2022 Martin and Johnstone.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Schlemm’s canal inner wall valve-like structure frequency and microsphere presence. (A) Eight primate eyes were perfused with 1 and 500 nm fluorescent microspheres. The entire circumference of each eye was divided into 250–500 µm segments. The total number of Schlemm’s canal (SC) inner wall valve-like structures (SIV) in each segment and those containing microspheres were recorded. The mean number of the SIV/segment was 1.16 ± 0.38. (B) Light microscopy demonstrates the distribution of 1-µm fluorescent (green) microspheres in each quadrant in one eye. (C) Chart demonstrates the percentage of SIV with microspheres compared to the total documented SIV in the four eyes. (D) Pie chart shows the total SIV in each quadrant. The chart also shows those with microspheres in the four eyes where 360° of the SC circumference was examined. The microsphere distribution in SIV was similar in each quadrant.