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. 2022 Jul 4;10:868029. doi: 10.3389/fcell.2022.868029

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Copyright © 2022 Martin and Johnstone.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Fluorescent microspheres in Schlemm’s canal inner wall valve-like structures. (A,B) Merged CD31 (red), DAPI (blue), and 500-nm fluorescent microsphere (green) channels. (A) Stack size is 40 μm and (B) is 43 µm. Schlemm’s canal (SC) inner wall endothelium forms a valve-like structure (SIV). The SIV arise from the SC inner wall endothelium (SCE) that forms the outer wall of the trabecular meshwork (TM). The funnel-shaped configuration extends into SC to form a cylindrical conduit attaching to the SC external wall at collector channels (CCs). The TM is filled with fluorescent microspheres. The microspheres fill the juxtacanalicular region with the SIV and the SIV connections with intrascleral channels (ISC) Supplementary Video S1 (A) #43LST12; (B) #43LIT4.