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. 2022 Jul 4;10:868029. doi: 10.3389/fcell.2022.868029

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Copyright © 2022 Martin and Johnstone.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Fluorescent microspheres entering and exiting Schlemm’s canal inlet valve-like structures. (A) Merged CD31 (red), DAPI (blue), and 500 nm fluorescent microsphere (green) channels. Two Schlemm’s canal (SC) valve-like structures (SIV) attach to the external wall of SC. The complex distal attachment creates a valve-like structure at the SC outlet (SOV). (B–D) Same SOV structure rotated around the Y-axis. (E–F) Merged IHC channels add the Nomarski view to better show the structure of the SIV and their attachments to the distal wall of SC Supplementary Video S2 #43LIT12.