TABLE 1.
Primary antibody | Dilution | Secondary antibody | Dilution |
---|---|---|---|
CD31, mouse antihuman CD31, endothelial cell; DAKO #M0823 | 1:20 | Alexa Fluor 647 | 1:200 |
Collagen type I (rabbit anticollagen type 1 pAb; calbiochem, #234167) | 1:40 | Alexa Fluor 555 | 1:200 |
Collagen type IV (rabbit antihuman collagen type IV; chemicon #AB748) | 1:20 | Alexa Fluor 555 | 1:200 |