TABLE 1.

Specific activities of T. kodakaraensis subtilisin and subtilisin E toward synthetic substrates^a

Substrate	T. kodakaraensis subtilisin		Subtilisin E
	Sp act (U/mg)	Relative activity (%)	Sp act (U/mg)	Relative activity (%)
AAPF	3,930	100	13,300	100
AAPL	955	24.3	2,820	21.2
AAPD	<40	<1.0	<40	<0.3

^aThe enzymatic activity was determined at pH 9.5 and 80°C for T. kodakaraensis subtilisin or pH 8.5 and 55°C for subtilisin E in the presence of 0.13 mM substrate and 5 mM CaCl₂. The experiment was performed in triplicate, and standard errors from the means were within 10% of the values reported.