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. 2022 Jul 4;13:862204. doi: 10.3389/fphar.2022.862204

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Copyright © 2022 Du, Li, Song, Wang, Dong, Zhan, Li and Liang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Interact effect of Compound C and Mdivi-1 on H/R induced mitochondrial fission in H9C2 cells. (A) Quantitative analysis of AMPK phosphorylation in cell lysate. (B,C) Quantitative analysis of Drp1 phosphorylation in cell lysate. (D–G) The mRNA expression of mitochondrial dynamics related factors including Fis1, Mff, Mfn1, and Mfn2 were detected by q-PCR. (H) Mitochondrial morphology were measured by TEM in H9C2 cells. The data were expressed as mean ± SEM (n = 6 per group). ^** p < 0.01, ^* p < 0.05 vs. H/R group; ^## p < 0.01, ^# p < 0.05 vs. H/R + Compound C group. ^▽▽ p < 0.01 vs. I/R + Mdivi-1 group.