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. 2022 Jul 4;13:912583. doi: 10.3389/fimmu.2022.912583

Figure 2.

Figure 2

ThGM cells upregulate RUNX3 in the CNS of mice with adoptive EAE in a T-bet-dependent manner. WT and Tbx21 -/- 2D2 naïve CD4+ T cells were differentiated into ThGM cells and transferred into Rag1 -/- mice (n = 10 mice/group). (A) Representative flow cytometry dot plots showing GM-CSF, IFN-γ, IL-17A, and T-bet expression by WT and Tbx21 -/- ThGM cells before adoptive transfer. (B) Clinical severity score of Rag1 -/- mice with adoptive EAE. (C) Representative histogram of T-bet, RUNX3, and RUNX1 expression by WT and Tbx21 -/- ThGM cells from the CNS of Rag1 -/- mice with adoptive EAE. (D) MFI for T-bet, RUNX1, and RUNX3 in WT and Tbx21 -/- ThGM cells from the CNS of Rag1 -/- mice with adoptive EAE. Data shown are mean ± SEM. For EAE, p-values were calculated using two-way ANOVA with Bonferroni’s multiple comparison correction. Parametric datasets were analyzed using unpaired Student’s t-test with Bonferroni’s correction; **p < 0.01, ***p < 0.001, NS, not significant.