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. 2022 Jul 4;10:914450. doi: 10.3389/fbioe.2022.914450

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Copyright © 2022 Nguyen, Wei, Nakada, Zhou and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cluster analysis identified 10 populations of cardiomyocytes in Fetal, CTL, AR_P1, MI_P28, and AR_P1MI_P28 hearts. (A,B) Cardiomyocyte snRNA-seq data were reduced to 10 dimensions with an autoencoder, processed via cluster analysis, visualized via UMAP, and displayed according to (A) experimental group and time point and (B) cluster (CM1–CM10). (C) The expression of genes that were explicitly associated with each cluster is displayed as a heat map. (D) The proportion of cardiomyocytes from each cluster is displayed for each experimental group and time point.