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. 2022 Jul 4;10:914450. doi: 10.3389/fbioe.2022.914450

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Copyright © 2022 Nguyen, Wei, Nakada, Zhou and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunofluorescence analysis confirming GRK5 expression in cardiomyocytes. Here, the expression of GRK5 was represented by the average GRK5 red-channel value (light intensity) that is overlapped with cardiac troponin (cTnT) blue-channel (foreground), which was calculated after adjusting GRK5 red-channel background. DAPI (blue) indicates nuclei. (A) Violinplot of GRK5 intensity in 118 staining images. Here, the horizontal bar and the star (*) indicate nonparametric Wilcoxon’s rank sum test between two sample groups. *p-value < 0.05. (B) Representative images of GRK5/cTnT/DAPI in each group.