Figure 4.

Training with HKCA en MDP induce differential type I IFN response upon re-stimulation. (A) Relative mRNA expression (2^ΔΔCT) of IFNB in THP-1 cells trained with heat-killed Candida albicans (HKCA; 10⁶ cells/mL), muramyl dipeptide (MDP; 50 µg/mL) or IFNβ and re-stimulated with 1 µg/mL LPS + 10% fetal calf serum for 24 hours. Fold change expression was calculated relative to the corresponding unstimulated control. (B, C) Type I IFN bioactivity quantified by the HEK IFN-α/β reporter cell assay in culture supernatants of (B) HKCA-, MDP- and IFNβ-trained THP-1 cells re-stimulated with 5 µg/mL poly I:C for 24 hours or (C) PBMCs from pSS patients stratified based on blood ISG expression and healthy controls (HC) stimulated with 5 µg/mL poly I:C or 10 ng/mL LPS for 24 hours. Symbols indicate the average of duplicates (C) or triplicates (A, B) and bars represent means or medians. Friedman test or repeated measures ANOVA was performed to compare groups. ns: not significant, *p<0.05, ***p<0.001.