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Signal stability upon multiple freeze-thaw-cycles. S1PR–aAb signal stability of aAb-positive serum was assessed with selected samples in the newly generated assays for (A) S1PR1–aAb, (B) S1PR2–aAb, and (C) S1PR3–aAb assay. Samples were subjected consecutively to four freeze and thaw cycles, and all measurements with one exception displayed signal intensities within a 20% range from the initial signal strength determined upon first thawing.
