Figure 1.

Overview of RNA-seq analysis. (A) Diagram showing the monensin treatment and sampling strategies. Fresh sporozoites of E. tenella-sensitive strain (S) and -resistant strain (R) were treated with 5 μg/ml monensin for 0, 2, and 4 h, respectively. Total RNAs were then subjected to RNA-seq. (B) PCA of all RNA-seq samples. (C) An upset-plot showing sets of differentially expressed genes after monensin treatment. Vertical bars show the number of intersecting genes between the comparison groups, denoted by the connected brown dots below the histogram. Horizontal bars show the size of DEGs between the comparison groups. The Venn diagram shows the size and overlapping situation of DEGs in the different groups.