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. 2022 Jul 4;13:934153. doi: 10.3389/fmicb.2022.934153

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Copyright © 2022 Zhang, Zhang, Si, Liu, Suo and Hu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Differential gene expression after monensin induction. The E. tenella-sensitive Houghton strain (ETH-S) underwent 16 generations of positive selection under increasing monensin concentration and then generated a monensin-resistant E. tenella line (ETH-R). (A) Volcano plot showing the difference in gene expression between ETH-R and ETH-S at 0 h of monensin treatment. Genes with an absolute fold change >2 and adjusted p < 0.01 were considered significantly differentially expressed (red dots). (B) Gene ontology enrichment analysis of differentially expressed genes between ETH-R and ETH-S at 0 h of monensin treatment. Clustered heatmaps show differentially expressed genes associated with ribosomal proteins (C) and mitochondrial proteins (D) between ETH-R and ETH-S at 0 h of monensin treatment. For heatmap drawing, gene expression levels were transformed into normalized log₂(TPM+1).