Figure 3.

Differential gene expression after 2 h of monensin treatment. Volcano plots showing differentially expressed genes in the monensin-sensitive ETH-S strain (A) and the monensin-resistant ETH-R strain (B) after 2 h of monensin treatment. Genes with an absolute fold change >2 and adjusted p < 0.01 were considered significantly differentially expressed (red dots). (C) Heatmaps showing differentially expressed genes associated with ubiquitin and proteasome in the monensin-sensitive ETH-S strain after 2 h of monensin treatment. For heatmap drawing, gene expression levels were transformed into normalized log₂(TPM+1).