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. 2022 Jul 4;15:896320. doi: 10.3389/fnmol.2022.896320

Figure 3.

Figure 3

Tool compound concentration-response curves on different Nav channel subtypes. (A) Representative fluorescence traces from DMSO, 1 μM TTX, or 10 μM tetracaine treated Nav1.2 spiking HEK cells (8 mM bath [K+]), Nav1.5 spiking HEK cells (6 mM bath [K+]), and Nav1.7 spiking HEK cells (4 mM bath [K+]). For Nav1.2 and Nav1.5 spiking HEK assays, the cells were stimulated at 2 Hz. Eight test pulses were applied before the 500 ms long pulse and only action potentials triggered by the third to the eighth pulses were plotted for these two Nav channel subtypes; for Nav1.7 spiking HEK cells, the cells were stimulated at 4 Hz and all 10 triggered action potentials are plotted. (B) Concentration-response curves of 3 non-selective Nav channel blockers (Amitriptyline, Tetracaine, Vixotrigine) and 3 subtype-selective Nav channel blockers (JNJ63955918, PF-05089771, Tetrodotoxin) on Nav1.2 (8 mM bath [K+]), Nav1.5 (6 mM bath [K+]), and Nav1.7 (8 mM bath [K+]). Error bars indicate SD based on measurements from 2 wells.