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Restriction map of the chromosomal region of M539 and subclones (the transposon is represented by a stem loop). cya and hypF represent the ORFs that were identified. The transposon is present only in pM539. TRHF06N, TRHF02, and TRHF01 primers were used in PCR. Restriction enzymes: A, ApaI; E, Eco147I; EV, EcoRV; N, NcoI.
