Figure 6. Knockout of Trim27 inhibits breast tumorigenesis but promotes lung metastasis in PyMT mice.

• A
  Comparison of the relative mRNA levels of TRIM27 in normal breast tissues (Normal, n = 113) and indicated breast tumors (Basal, n = 143; Her2, n = 67; LumA, n = 427; LumB, n = 190) in the TCGA database. The number of biological replicates are shown as indicated. P < 0.0001 (one‐way ANOVA).
• B
  Analysis of the correlation between ULK1 and TRIM27 expression in the CPTAC proteomics database. P = 0.0027 (Pearson’s correlation method).
• C, D
  Western blot analysis of ULK1 and TRIM27 levels from tumor tissues (T), Pericarcinomatous tissues (P), and their matched surrounding normal tissues (N). GAPDH was used as a loading control (C). A total of 34 samples of human breast cancer were classified into nine groups based on the expression of ULK1 and TRIM27 in tumor tissues compared with surrounding normal tissues (D).
• E
  Schematic diagram of Trim27^−/− PyMT and Trim27^+/− PyMT mice breeding, Trim27^+/− PyMT mice as a littermate control.
• F
  Representative tumors in MMTV‐PyMT mice of the indicated Trim27 genotypes. Scale bar, 5 mm.
• G
  Western blot analysis of indicated proteins in Trim27^−/− PyMT or Trim27^+/− PyMT mice in primary breast tumors. Actin was used as a loading control.
• H
  Kaplan–Meier tumor‐free survival curves of Trim27^+/− PyMT (median = 75 days, number = 20), or Trim27^−/− PyMT mice (median = 95 days, number = 16). P < 0.0001 (Mantel–Cox test).
• I–K
  Pulmonary surface nodules and hematoxylin and eosin (H&E) staining in Trim27^+/− PyMT mice and Trim27^−/− PyMT mice (I and J). Scale bar, 1 mm (K). Bar chart showing quantification for the number of metastatic nodules per lung lope (Trim27^+/−, n = 6 mice; Trim27^−/− n = 6 mice), when compared at the same final timepoint. Each symbol represents a mouse; Data are presented as mean ± SD from three independent experiments; ***P < 0.001 (unpaired student's t‐test).

Source data are available online for this figure.