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. 2022 Jun 13;41(14):e110611. doi: 10.15252/embj.2022110611

Figure 3. Genome‐wide CRISPR screen establishes sensitizers to DT‐061 and iHAP1.

Figure 3

  1. Schematic of the experimental setup and results of CRISPR screen. Genes were ranked based on their normZ score. The top hits which were further analyzed are highlighted.
  2. Validation of CRISPR screen results using RNAi depletion followed by SRB growth assay. Cells were treated with DMSO (Ctr) or DT‐061 5.5 µM or iHAP1 0.5 µM.
  3. Incucyte growth assay after RNAi depletion of indicated proteins alone or in combination with DMSO or 5.5 μM DT‐061 or 0.5 μM iHAP1 treatment.

Data information: (B and C) Mean and SD of three independent experiments are shown.

Source data are available online for this figure.