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. 2022 Jun 13;41(14):e110611. doi: 10.15252/embj.2022110611

Figure EV3. Compound titration and validation of CRISPR screen results.

Figure EV3

  1. Viability of RPE1‐hTERT P53−/− FLAG Cas9 cells with increasing concentrations of iHAP1 or DT‐061 to determine LD20. Mean and SD of three biological replicates is shown.
  2. Protein depletion efficiency after RNAi‐treatment. Actin was used as loading control. The experiment was performed three times.
  3. Sensitivity of HeLa Rod1 KO cells to 0.5 µM iHAP1. Western blot to confirm Rod1 depletion levels (left) and growth curves for the indicated conditions (right).
  4. Sensitivity of HeLa Rod1 KO to DT‐061 or iHAP1 determined using SRB assay.

Data information: (C and D) Mean and SD of three independent experiments are shown.