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. 2022 Jun 13;41(14):e110611. doi: 10.15252/embj.2022110611

Figure EV4. Analysis of mitotic phenotypes in cells treated with DT‐061 or iHAP1.

Figure EV4

  1. Representative spinning‐disk confocal microscopy time‐series of mitosis in U2OS cells stably expressing H2B‐GFP/mCherry‐α‐tubulin following indicated treatments. Scale bar indicates 10 µm.
  2. Representative images of U2OS mitotic spindles in metaphase following indicated treatments, immunostained with α‐tubulin antibody. DNA was counterstained with DAPI (cyan). Scale bar indicates 10 µm.
  3. Illustration describing the method used for measuring the amount of total, spindle and astral microtubules.
  4. Quantification of the number of microtubules in metaphase‐arrested mitotic cells following indicated treatments. The mean and SD are plotted from three independent experiments (Total no. of cells = 48 (DMSO), 45 (30 µM DT‐061+APC/C in.) and 48 (2 µM iHAP1+APC/C in.)). P‐values were calculated using Student’s t‐test or Mann–Whitney U test (unpaired, two‐tailed). See Materials and Methods for more details. ns, not significant, ****P < 0.0001.