Figure 7. DT‐061 disrupts the structure of Golgi and ER in H358 cells.

• A, B
  Time‐lapse imaging of H358 cells expressing a marker for the Golgi (A) or the ER (B). Cells were treated with DT‐061 at time 0. (B, low panel). For (A) 177 cells were analyzed from one experiment and all displayed dispersal of the Golgi marker. Arrow indicates addition of compound.
• C
  Time needed for ER translocation into the nucleus after drug treatment. Median (red line): 160 min. Number of cells analyzed per condition shown on top.
• D
  Percentage of cells with ER nuclear translocation after drug treatment. Number of cells analyzed per condition shown on top.

Data information: (A–D) Experiments were repeated at least twice except filming of the Golgi marker which was done once. Stills show a representative cell for each treatment condition. Cells were treated with DMSO (Ctrl) or DT‐061 at the concentration stated in the figure. (A and B) Scale bar indicates 20 µm.

Source data are available online for this figure.