Skip to main content
. 2022 Jun 13;41(14):e110611. doi: 10.15252/embj.2022110611

Figure 8. DT‐061 affects sphingolipid biosynthesis in Golgi and ER.

Figure 8

  1. Schematic illustration of cellular sphingolipid biosynthesis in ER and Golgi.
  2. Increase in the molar quantity of sphingolipid classes assessed in MCF7 cells after feeding with the precursor sphingosine. MCF7 cells were co‐treated with 15 μM DT‐061, 1.5 μM iHAP1, 3 µg/ml Brefeldin A or vehicle (DMSO). The determined molar quantities of sphingolipid classes were normalized to that of PC. The presented values represent averages of five independent experiments, and statistical analysis was performed using an independent t‐test. *P < 0.05, **P < 0.01, ***P < 0.001. Cer: ceramide, HexCer: hexosylceramide, PC: phosphatidylcholine and SM: sphingomyelin.

Source data are available online for this figure.