Fig. 3.

Tax4Fun functional prediction analysis of differential bacteria associated with hepatic Kupffer cell (KC) accumulation; tumor inhibition was noted in the three groups. (A) Tax4Fun functional prediction in the control, Vanc, and Coli groups, as shown as a heatmap. (B) Enrichment of immune system, environmental information, and cellular process pathways were compared in the control and Coli groups (dot size symbolizes the p-value). (C) Enrichment of immune system, environmental information, and cellular process pathways were compared in the control and Vanc groups. (D) Hematoxylin and eosin staining images and quantification of hepatic KCs from different gut flora backgrounds (n=5 per group, ×200). (E) Analysis of the correlation between KCs and Proteus mirabilis or Bacteroides vulgatus.

Control group, untreated; Vanc group, vancomycin; Coli group, colistin; ASC group, a mix of ampicillin, colistin, and streptomycin. *p<0.05, ^†p<0.01.