Figure 7.

Compound 5 and quinacrine treatment of ScN2a RML cells. (A,B) Representative Western blot images of PrP^Sc, total PrP, and β-actin in lysates from ScN2a RML cells treated with (A) vehicles, quinacrine (1 μM), or compound 5 (10 μM) and (B) vehicles or a dose gradient of compound 5 and quinacrine (2–10 μM and 0.2–1 μM, respectively). (C) Pairwise dose–response data (left) and isobologram synergy plot (right) were presented for the combination treatment. Dose–response data are derived from a nonlinear regression of 3 experimental replicates, plotted with mean ± SD. The diagonal line of the isobologram represents the additive line. EC_50,add dot corresponds to the calculated, theoretical paired value, when assuming an additive interaction between the two drugs. EC_50,mix represents the paired value of drug concentrations assessed or synergism. The interaction index (γ) was calculated, and it corresponds to a value of 0.19.