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. 2022 Jun 27;65(13):9050–9062. doi: 10.1021/acs.jmedchem.2c00270

Figure 3.

Figure 3

Effects of AMPs on P. aeruginosa CF isolate and PAO1 biofilm in ASM. P. aeruginosa CF isolates and PAO1 were grown in a 24-well plate with ASM for 3 days prior to exposure to different concentrations of AMPs for 24 h. Disruption of the biofilm was conducted by a 1 h incubation with cellulose. Viability was measured by a 2 h incubation with 0.02% resazurin. A plate reader with an excitation wavelength of 530 nm and an emission wavelength of 590 nm was used to measure fluorescence: (A) CF isolate 24, (B) CF isolate 46, (C) CF isolate 82, and (D) PAO1. The data are presented as means ± standard errors. The statistical significance from untreated biofilm was determined by ANOVA.