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. 2022 Jun 27;65(13):9050–9062. doi: 10.1021/acs.jmedchem.2c00270

Figure 5.

Figure 5

AMP antibiofilm activity in the presence of CF sputum. PAO1 was grown on a slide chamber with the sputum supernatant [10% (v/v)] for 48 h prior to exposure to a concentration of 10 μM for 1 h. Biofilms were stained with a live/dead cell kit for 1 h prior to fluorescence imaging: (A) untreated sample, (B) LL-37, (C) Seg5D, (D) Seg6D, and (E) Amp1D. Images were taken using an Olympus FV1000 confocal microscope [60× objective lens (oil), 10 μm scale bars]. (F) Biofilm viability according to fluorescence intensity. Data were analyzed using Olympus Fluoview (version 4.1) and ImageJ. (G) The viability of biofilms was checked using resazurin. The statistical significance from untreated biofilm was determined by ANOVA.