Figure 5.

Antagonism of NECA-induced A_2A receptor-mediated cAMP accumulation as measured by a GloSensor assay. Ability of the A_2A receptor antagonist, ZM 241385, fragment library hits (A,B,D,E) or kinase library hits (C,F) to inhibit the accumulation of cAMP as induced by an effective 80% concentration (EC₈₀) of NECA (500 nM) or effective 20% concentration (EC₂₀) of NECA (3 nM). Data are expressed as a percentage of the maximum NECA response and represent the mean ± SEM of at least three independent experiments performed in technical duplicate.