TABLE 1.
Susceptibility profile and genomic features of KPC‐2‐producing Klebsiella pneumoniae strain isolated from an infected dog in Brazil
| Susceptibility profile a | |
| Amoxicillin/clavulanic acid | R |
| Aztreonam | R |
| Cefotaxime | R |
| Ceftriaxone | R |
| Ceftazidime | R |
| Ceftiofur | R |
| Cefoxitin | R |
| Cefepime | R |
| Ertapenem | R |
| Imipenem (MIC mg/L) | R (>32) |
| Meropenem (MIC mg/L) | R (>32) |
| Amikacin (MIC mg/L) | R (64) |
| Gentamicin (MIC mg/L) | R (>256) |
| Sulfamethoxazole/trimethoprim | R |
| Nalidixic acid | R |
| Enrofloxacin | R |
| Ciprofloxacin | R |
| Chloramphenicol | R |
| Tetracycline | S |
| Colistin (MIC mg/L) | S (2) |
| Molecular epidemiology | |
| MLST (ST/CG) b | 11/258 |
| K‐locus | KL15 |
| wzi | 50 |
| Serotype | O4 |
| Resistome | |
| β‐lactams | bla KPC−2, bla CTX‐M−15, bla LAP−2, bla OXA−1, bla SHV−11 |
| Quinolones | aac(6')‐Ib‐cr, oqxA, oqxB, qnrS1, gyrA (S83I), parC (S80I) |
| Aminoglycosides | aac(3)‐IIa, aadA2, aph(3')‐Ia |
| Sulfamethoxazole | sul1 |
| Trimethoprim | dfrA12 |
| Fosfomycin | fosA |
| Macrolides | mphA |
| Chloramphenicol | catB4 |
| Virulome | |
| Yersiniabactin siderophore | ybt, fyuA, irp |
| Plasmidome | |
| Inc‐type [size, kb] c | IncFIB(K) [168], IncN [54], Col4401‐like [76] |
| GenBank accession number | JABSUB000000000.1 |
a
Susceptibility profiles were determined using the CLSI guideline (CLSI, 2020). For ceftiofur, enrofloxacin and colistin, resistance profiles were determined using veterinary CLSI (CLSI, 2018) and EUCAST 2021 (https://www.eucast.org/) guidelines, respectively.
b
MLST, Multi‐Locus Sequence Typing; ST, sequence type; CG, clonal group.
c
The IncFIB(K) plasmid, named pPVT01_P1, harboured bla CTX‐M‐15, aac(3)‐IIa, aadA2, aph(3')‐Ia, mphA, sul and dfrA12, whereas Col4401‐like plasmid (pPVT01_P2) harboured bla OXA‐1, bla LAP‐2, qnrS1 and aac(6')‐Ib‐cr resistance genes.