Autologous red blood cells*	544 ml
Sodium chloride 0.9% (Fresenius Kabi Nederland B.V., Zeist, Netherlands)	600 ml
Glucose 5% (Baxter BV, Utrecht, Netherlands)	20 ml
Human albumin 20% (Albuman 200 g/l, Sanquin Plasma Products B. V., Amsterdam, Netherlands)	200 ml
Sodium bicarbonate 8.4% (B. Braun Melsungen AG, Melsungen, Germany)	16 ml
Calcium gluconate 10% (B. Braun )	10 ml
Amoxicillin/clavulanic acid (Sandoz B.V., Almere, Netherlands)	1200 mg (in 20ml water solution)
Mannitol (Baxter B.V.)	32 mg
Creatinine (Sigma‐Aldrich, Zwijndrecht, Netherlands)	160 mg
Insulin (Novo Nordisk A/S, Bagsværd, Denmark)	16 U
Sterofundin ISO (B. Braun)	Added 20 ml every hour
Amoxicillin/clavulanic acid	Added 192 mg (3.2 ml) every hour
Calcium gluconate 10%	Added 3 ml if calcium <1.1 mmol/l
Glucose 5%	Added 13 ml if glucose <4.6 mmol/l

^*Autologous blood from each pig was obtained and mixed with 25,000 units of heparin (LEO® pharma, Ballerup, Denmark). Whole blood was depleted of leukocytes using a leukocyte filter (BioR 02 plus BS PF, Fresenius Kabi, Zeist, the Netherlands) after which a red blood cells (RBC) concentrate was obtained by centrifuging, removing supernatant plasma, washing with phosphate‐buffered saline, centrifuging again, and separating the pure RBCs.