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. Author manuscript; available in PMC: 2022 Sep 10.
Published in final edited form as: Sci Total Environ. 2022 Jun 1;838(Pt 1):155713. doi: 10.1016/j.scitotenv.2022.155713

Fig. 2.

Fig. 2.

Intranasal instillation of Cr(VI) for 12 weeks promoted CXCL5 expression in BALB/cJ mice. BALB/cJ mice were treated intranasally with zinc chromate (50 μg/50 μL/ mouse) or saline solution (vehicle for the control group) once a week. After 12 weeks, mice were euthanized, and blood and lung tissues were collected for further examination. PBMCs were separated using Ficoll-Paque. (A) Lung tissues were fixed and embedded using formalin. Immunohistochemical (IHC) staining was performed to detect CXCL5 and its receptor CXCR2. Magnification: 200×. (B) Lung tissues were used to extract RNAs. The expression levels of CXCL5 and CXCR2 was determined using RT-qPCR. (C) PBMCs were isolated from blood samples of mice, the expression of CXCL5 and CXCR2 was analyzed using RT-qPCR as above. ****, p < 0.0001 compared to control group without Cr(VI) intranasal instillation.