FIG. 5.

Transmission electron micrographs of PL and GR from S. littoralis after 1 h of incubation in cytolytic extracts of X. nematophila F1/1. n, nuclei; arrowhead, vesicle of ER; arrow, perinuclear cisterna. (A) Control, sterile LB. (B) C1 extract diluted 1/3 (vol/vol) in PBS. Note the dilated vesicles of ER (arrowheads) and perinuclear cisternae (arrows). (C) C2 extract diluted 1/3 (vol/vol) in PBS. Hemocytes are shrunken cells with a dense hyaloplasm. Chromatin is condensed in rounded masses, especially in PL (compare with panel A). Bar = 1.5 μm.