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. 2022 Jul 18;24:51. doi: 10.1186/s13058-022-01542-y

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Enhanced soft agar growth with combined Dusp4 loss and Trp53 (p53) deletion. A Crystal violet 2D culture assay of PMECs transduced with CRISPR/Cas9 constructs targeting Trp53 (exon 7 or exon 5) or scramble control (Parent) and selected with 10uM nutlin-3a for 1 week, or DMSO control. Trp53 CRISPR transductants demonstrate insensitivity to MDM2 inhibition, verifying loss of p53 function. B Soft agar colony formation assay for Dusp4FLOX or Dusp4NULL PMECs transduced with control sgRNA or Trp53 sgRNA. C Western blot analysis of derivative PMECs