Fig. 5.

Combined Dusp4 and p53 loss induces multinucleation and eventual escape from replication stress. A Representative images of polyploidy/multinucleation in Dusp4 NULL cells. B Quantification of nuclear content in primary mammary epithelial-derived cell lines with various genetic alterations. C Representative images of Dusp4NULL cells stained with DAPI to visualize nuclear DNA content. D Cells were labeled with EdU and counterstained with DAPI and analyzed by flow cytometry to analyze DNA synthesis. Representative flow cytometry gating is shown. E S phase analysis by EdU *Tukey’s post hoc p < 0.05** p < 0.001. F G2 phase analysis demonstrating increased G2 phase in Dusp4NULL p53Δ and DPM cells. G Whole-cell lysates were immunoblotted for replication stress marker pCHK1