Fig. 1.
The effect of WJ-MSCs ECM on human umbilical vein endothelial cells (HUVECs) angiogenesis ability. a, b Immunofluorescently stained for collagen I (Col I) of WJ-MSCs or WJ-MSCs ECM (magnification: × 400). n = 3. c Immunofluorescently stained for Fibronectin of WJ-MSCs ECM (magnification: × 400). n = 3. d Immunofluorescently stained for Laminin of WJ-MSCs ECM (magnification: × 400). n = 3. e–h, m Matrigel tube formation assay images and quantitative analysis of cumulative tube length. Bar = 50 μm. Mean ± S.E.M., n = 3. i–l, n: Transwell for HUVECs migration assay and quantitative analysis of migrated HUVECs numbers. Bar = 50 μm. Mean ± S.E.M., n = 3. The P value was calculated using one-way ANOVA and independent samples t-test. *P < 0.05, **P < 0.01 versus control