Table 5.
Variant Detection in Mixtures Prepared from Two Samples: 20A and 20B
Mix | % 20A specimen | % 20B specimen | Common variants detected (4 total∗) | 20A variants detected (6 total†) | 20B variants detected (10 total‡) |
---|---|---|---|---|---|
1 | 0 | 100 | 4 | 0 | 10 |
2 | 1 | 99 | 4 | 4 | 10 |
3 | 5 | 95 | 4 | 6 | 10 |
4 | 10 | 90 | 4 | 6 | 10 |
5 | 50 | 50 | 4 | 6 | 10 |
6 | 100 | 0 | 4 | 6 | 0 |
20A, WA-UW-20236 TM; 20B, WA-UW-19433 TM.
Expected variants common to both samples (n = 4): C241T, C3037T, C14408T, and A23403G.
Variants in 20A sample (n = 6): C4633T, C10965T, T14643C, A20268G, C22482T, and C28854T.
Variants in 20B sample (n = 10): G2144T, G3824A, G13348T, C15933T, G16968T, T19839C, G28083T, and GGG(28881 to 28883) AAC.