Key resources table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| PE Mouse anti-human CD31 (clone WM59) | BD Bioscience | Cat 555446 |
| BV421 Mouse anti-human CD45 (clone 2D1) | Biolegend | Cat 368522 |
| BV421 mouse anti-human CD31 (clone WM59) | Biolegend | Cat 303124 |
| Fitc mouse anti-human CD14 (clone 63D3) | Biolegend | Cat 367116 |
| Pe mouse anti-human CD26 (clone BA5b) | Biolegend | Cat 302706 |
| PE/Cyanine 7 mouse anti-human CD36 (clone 5-271) | Biolegend | Cat 336222 |
| Goat anti-mouse MRC1/CD206 (polyclonal) | R&D systems | Cat AF2535 |
| PE mouse anti-human/mouse MAF (clone symOF1) | ThermoFisher Scientific | Cat 12-9855-42 |
| APC/Cyanine 7 anti-human CD45 (clone HI30) | Biolegend | Cat 304014 |
| PE/Cyanine 7 mouse anti-human CD34 (clone 581) | Biolegend | Cat 343416 |
| BV421 mouse anti-human CD38 (clone HB-7) | Biolegend | Cat 356618 |
| Human TruStain FC Blocker | Biolegend | Cat 422302 |
| Pe/Cyanine 7 rat anti-mouse CD31 (clone 390) | Biolegend | Cat 102418 |
| Alexa 488 rat anti-mouse CD45 (clone 30-F11) | Biolegend | Cat 103122 |
| BV421 rat anti-mouse CD45 (clone 30-F11) | Biolegend | Cat 103134 |
| APC/Cyanine 7 rat anti-mouse CD45 (clone 30-F11) | Biolegend | Cat 103116 |
| PE mouse anti-mouse CD32B (clone AT130-2) | ThermoFisher Scientific | Cat 12-0321082 |
| BV421 rat anti-mouse MRC1/CD206 (clone C068C2) | Biolegend | Cat 141717 |
| PE/Cyanine 7 rat anti-mouse Ly6A/E (clone D7) | Biolegend | Cat 108114 |
| APC mouse anti-mouse CD32B (clone AT130-2) | ThermoFisher Scientific | Cat 17-0321-82 |
| APC rat anti-mouse CD68 (clone FA-11) | Biolegend | Cat 137008 |
| Alexa 488 rat anti-mouse CD31 (clone 390) | Biolegend | Cat 102414 |
| TruStain FcX rat anti-mouse CD16/32 (Clone 93) | Biolegend | Cat 101320 |
| APC rat anti-mouse CD45R/B220 (clone RA3-6B2) | Biolegend | Cat 103212 |
| Alexa 488 rat anti-mouse CD24 (clone M1/69) | Biolegend | Cat 101816 |
| PE/Cyanine 7 rat anti-mouse CD45 (clone 30-F11) | Biolegend | Cat 103114 |
| PE rat anti-mouse CD20 (clone SA275A11) | Biolegend | Cat 150409 |
| PE/Cyanine 7 rat anti-mouse F4/80 (clone BM8) | Biolegend | Cat 123113 |
| BV421 rat anti-mouse CD68 (clone FA-11) | Biolegend | Cat 137017 |
| Alexa 700 rat anti-mouse CD45 (clone 30-F11) | Biolegend | Cat 103128 |
| Alexa 647 rat anti-mouse Cdh5 (clone11D4.1) | BD Bioscience | Cat 562242 |
| Alexa 647 rat anti-mouse Cdh5 (clone BV13) | Biolegend | Cat 138005 |
| Rabbit anti-human CD34 (polyclonal) | Millipore Sigma | Cat HPA036722 |
| Mouse anti-human CD34 (clone QBEnd 10) | Dako | Cat M716501-2 |
| Goat anti-mouse Lyve1 (polyclonal) | R&D systems | Cat AF2125 |
| Goat anti-human Lyve1 (polyclonal) | R&D systems | Cat AF2089 |
| Goat anti-human VE-Cadherin (polyclonal) | R&D systems | Cat AF938 |
| Rabbit anti-human CD14 (polyclonal) | Millipore Sigma | Cat HPA001887 |
| Mouse anti-human ENG (clone CL1912) | Millipore Sigma | Cat AMAB90925 |
| Rat anti-mouse Endomucin (clone V.7C7) | Santa Cruz | Cat sc-65495 |
| Rabbit anti-human/mouse Aqp1 (polyclonal) | Millipore Sigma | Cat HPA019206 |
| Mouse anti-human MRC1/CD206 (clone 685645) | R&D systems | Cat MAB25341 |
| Mouse anti-human/mouse Glutamine Synthetase | BD Bioscience | Cat 610518; RRID AB_397880 |
| Rabbit anti-human/mouse Cyp2E1 (polyclonal) | Millipore Sigma | Cat AB1252 |
| Alexa 594 Rat anti-mouse E-cadherin (clone DEMCA-1) | Biolegend | Cat 147306 |
| Alexa 488 donkey Anti-rabbit (polyclonal) | ThermoFisher Scientific | Cat A21206 |
| Alexa 594 donkey anti-rat (polyclonal) | ThermoFisher Scientific | Cat A21209 |
| Alexa 555 donkey anti-mouse (polyclonal) | ThermoFisher Scientific | Cat A31570 |
| Alexa 647 donkey anti-goat (polyclonal) | Jackson Immuno Research Laboratories | Cat 705-605-147; RRID: AB_2340437 |
| Deposited data | ||
| Single cell of temporal analysis of mouse liver endothelium | GEO | GSE174209 |
| Single cell of hematopoietic and endothelial cells at postnatal day P15 of Control and c-Maf deficient endothelial mice | GEO | GSE174208 |
| Bulk RNA-seq from adult mouse endothelium | GEO | GSE172360 |
| Single cell from human CD31+ CD45neg sorted liver cells | GEO | GSE172361 |
| Experimental models: Cell lines | ||
| Primary Human Vascular Umbilical Endothelial Cells | Laboratory of Shahin Rafii | NA |
| Lentivirus packaging cell line: 293T cells | ATCC | Cat CRL-3216 |
| Experimental models: Organisms/strains | ||
| Mouse: C57BL/6J | The Jackson Laboratories | Cat 000664 |
| Mouse: VE-cadherin Cre/ERT2 | Laboratory of Ralf H Adams | MGI: 3848982 |
| Mouse: Maf flox/flox | Laboratory of Carmen Birchmeier | MGI: 5316775 |
| Oligonucleotides | ||
| Table S5 | NA | NA |
| Recombinant DNA | ||
| Tamoxifen inducible human Maf (c-Maf) | Vectorbuilder | Cat VB170606-1099bva |
| Software and algorithms | ||
| R v4.0.2 | R | NA |
| Prism v.5 | GraphPad | RRRID: SCR_002798 |
| FACS Diva | BD Bioscience | NA |
| Zen blue | Nikon | NA |
| Seurat (v 3.2.3) | Butler et al., 2018; Stuart et al., 2019 | NA |