Fig. 7.

Release of proteins from the non-clinical and clinical CTB-ACE2 chewing gums and GFP chewing gum. Protein release was evaluated in the homogenate at 0, 10, and 20 min. After 20 min of simulated chewing, 99.6 ± 4.9 μg CTB-ACE2 (27% release) and 313.0 ± 6.8 μg (42% release) was released from the 365.8 ± 18.0 μg and 752.7 ± 3.4 μg tablet, respectively. The 448 μg GFP gum released 243.9 ± 7.4 μg (54% release) after 20 min of simulated chewing. There is a significant difference between the CTB-ACE2 released at 0 and 20 min for both doses of chewing gum (****p-value < 0.00001). Similarly, there is significant difference of GFP released from the GFP tablet at 0 and 20 min (****p-value < 0.00001); data were analyzed by a one-way ANOVA test. The data represents mean ± standard deviation (SD) (n = 4).