| Antibodies |
|
| HRP conjugated anti-GFP polyclonal primary antibody |
Millipore Sigma |
Cat#GERPN1236; RRID:AB_771429 |
|
| Bacterial and virus strains |
|
| BL21-DE3 |
Millipore Sigma |
Cat#CMC0016 |
| BL21-DE3 LOBSTR |
Kerafast |
Cat#EC1002 |
|
| Chemicals, peptides, and recombinant proteins |
|
| ΔIBB-importin α1 construct |
This paper |
Cingolani_001 |
| Importin α1/β heterodimer |
This paper |
Cingolani_002 |
| GST-TDP-43 NTD-NLS-RRM1 wild-type |
This paper |
Cingolani_003 |
| GST-TDP-43 NTD-NLS-RRM1 T88E (pT88) |
This paper |
Cingolani_004 |
| GST-TDP-43 NTD-NLS-RRM1 S91E (pS91) |
This paper |
Cingolani_005 |
| GST-TDP-43 NTD-NLS-RRM1 S92E (pS92) |
This paper |
Cingolani_006 |
| GST-TDP-43 NTD-NLS-RRM1 T88E, S91E, S92E (pT88+pS91+pS92) |
This paper |
Cingolani_007 |
| GST-TDP-43 NTD-NLS |
This paper |
Cingolani_008 |
| GST-TDP-43 NLS-RRM1 |
This paper |
Cingolani_009 |
| Ampicillin |
ThermoFisher Scientific |
CAS#69-53-4 Cat#11593027 |
| Kanamycin sulfate |
ThermoFisher Scientific |
CAS#25389-94-0 Cat#15160054 |
| Chloramphenicol |
ThermoFisher Scientific |
CAS#56-75-7 Cat#227925000 |
| 3,3’,5,5’-Tetramethylbenzadine (TMB) |
abcam |
CAS#54827-17-7 Cat#ab171523 |
| Isopropylthio-β-galactoside (IPTG), >99% |
ThermoFisher Scientific |
CAS#367-93-1 Cat#15529019 |
| Sodium Chloride (NaCl), >99% |
ThermoFisher Scientific |
CAS#7647-14-5 Cat#A12313.0I |
| Tris base, >98% |
ThermoFisher Scientific |
CAS#77-86-1 Cat#J22675.36 |
| 2-mercaptoethanol (BME), 14.3 M, >99% |
Millipore Sigma |
CAS#60-24-2 Cat#M6250 |
| Tween-20 |
Millipore Sigma |
CAS#9005-64-5 Cat# P1379 |
| Hydrochloric acid (HCl), 12 N |
ThermoFisher Scientific |
CAS#7647-01-0 Cat#NC004378 |
| Dulbecco’s Phosphate buffered saline (PBS), 10× |
Millipore Sigma |
CAS#7647-14-5 Cat#D1408 |
| Ethylenedinitrilotetraacetic acid disodium salt (EDTA), >99%, |
Millipore Sigma |
CAS#6381-92-6 Cat#E5134 |
| Imidazole, >99% |
Millipore Sigma |
CAS#288-32-4 Cat#814223 |
| High density nickel resin |
GoldBio |
Cat#H-320-5 |
| GST agarose resin |
GoldBio |
Cat#G-250-5 |
|
| Critical commercial assays |
|
| Pierce BCA Protein Assay Kit |
ThermoFisher Scientific |
Cat#23227 |
| Natrix Crystallization Screen |
Hampton Research |
HR2-116 |
| Natrix 2 Crystalization Screen |
Hampton Research |
HR2-117 |
| Crystal Screen |
Hampton Research |
HR2-110 |
| Index Screen |
Hampton Research |
HR2-144 |
|
| Deposited data |
|
| Raw and analyzed X-ray data for TDP-43 NTD-NLS+ΔIBB-importin α1 |
This paper |
Mendely Data: https://data.mendeley.com/datasets/mpkdxc4hsj/
|
| TDP-43 NTD-NLS+ΔIBB-importin α1 PDB |
This paper |
PDB: 7N9H |
|
| Recombinant DNA |
|
| Vector: pET-30a |
EMD biosciences/Millipore Sigma |
Cat#69909 |
| Vector: pGEX-6p-1 |
Amersham/Millipore Sigma |
Cat# GE28-9546-48 |
| Vector: pETduet-1 |
EDM biosciences/Millipore Sigma |
Cat#71146 |
|
| Software and algorithms |
|
| ImageJ |
(Abramoff et al., 2004) |
N/A |
| PyMol |
(DeLano, 2002) |
N/A |
| HKL2000 |
(Otwinowski and Minor, 1997) |
N/A |
| Phaser |
(McCoy, 2007) |
N/A |
| Phenix.refine |
(Liebschner et al., 2019) |
Version 1.19.2 |
| Phenix.polder |
(Liebschner et al., 2017) |
N/A |
| COOT |
(Emsley and Cowtan, 2004) |
N/A |
| ATSAS |
(Franke et al., 2017) |
N/A |
| RAW |
(Hopkins et al., 2017) |
Version 2.1.1 |
| DENSS |
(Grant, 2018) |
N/A |
| Chimera |
(Pettersen et al., 2004) |
N/A |
| FoXS Server |
(Schneidman-Duhovny et al., 2010) |
N/A |
| GraphPad Prism |
Non-linear regression: one-site specific binding curve |
Version 9.0.0 |
| Chromlab |
Bio-Rad |
N/A |
| SEDFIT |
(Schuck, 2000) |
Center for Information Technology, NIH |
| SEDNTERP |
(Laue et al., 1992) |
N/A |
| GUSSI |
UTSouthwestern Medical Center |
N/A |
| PDBsum |
(Laskowski, 2009) |
N/A |
| PISA |
(Krissinel and Henrick, 2007) |
N/A |
| Pyemma |
(Scherer et al., 2015) |
Version 2.5.7 |
| Amber |
(D.A. Case et al., 2018) |
Version 18 |
| SHAKE algorithm |
(Elber et al., 2011) |
N/A |
| PCCA++ algorithm |
(Roblitz et al., 2013) |
N/A |
| VMD |
(Humphrey et al., 1996) |
N/A |
