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(a) Map of part of the nif region in C. tepidum; (b) map of the aadA streptomycin-spectinomycin resistance cassette from pHP45Ω; (c) map of the aacC1 gentamicin resistance cassette from pMS266; (d) map of the ermC-cat erythromycin and chloramphenicol resistance cassette from pRL409. The positions of some of the primers discussed in the text are also shown.
