. 2001 Jun;67(6):2538–2544. doi: 10.1128/AEM.67.6.2538-2544.2001

TABLE 1.

Plasmids used in this study

Name	Size (kb)	Relevant characteristics	Phenotype^a	Source or reference
pMS266	3.7	Contains a 1.1-kb aacC1 gentamicin resistance cassette	Ap^r Gm^r	1
pHP45Ω	4.4	Contains a 2.1-kb aadA streptomycin and spectinomycin resistance cassette	Ap^r Sm^r Sp^r	16
pRL409	5.4	Contains a 2.8-kb ermC-cat erythromycin and chloramphenicol resistance cassette	Ap^r Em^r Cm^r	7
pTN1	5.6	Derived from pUC19; contains a 2.93-kb fraction of the C. tepidum nif region	Ap^r	This study
pTN1G4	6.7	Similar to pTN1, but nifD is interrupted by the aacC1 cassette from pMS266	Ap^r Gm^r	This study
pTN2G1	4.8	Similar to pTN1G4 but contains only a 1.08-kb fraction of the C. tepidum nif region	Ap^r Gm^r	This study
pTN3G11	4.0	Similar to pTN1G4 but contains only a 0.29-kb fraction of the C. tepidum nif region	Ap^r Gm^r	This study
pTN1S3	7.7	Similar to pTN1, but nifD is interrupted by the aadA cassette from pHP45Ω	Ap^r Sm^r Sp^r	This study
pTN1CE1	8.4	Similar to pTN1, but nifD is interrupted by the ermC-cat cassette from pRL409	Ap^r Em^r Cm^r	This study

Ap^r, ampicillin resistant; Gm^r, gentamicin resistant; Sm^r, streptomycin resistant; Sp^r, spectinomycin resistant; Em^r, erythromycin resistant; Cm^r, chloramphenicol resistant.