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Transformation with 10 μg of DNA with various lengths of homologous regions
| DNA | Total length of homologous DNA (kb) | Transformation frequencya |
|---|---|---|
| AhdI-digested pTN1G4 | 2.93 | (1.3 ± 0.4) × 10−7 |
| EcoRI-digested pTN1G4 | 2.93 | (4 ± 1) × 10−7 |
| AhdI-digested pTN2G1 | 1.08 | (1.1 ± 0.3) × 10−7 |
| EcoRI-digested pTN2G1 | 1.08 | (7 ± 3) × 10−9 |
| AhdI-digested pTN3G11 | 0.29 | 0 |
| EcoRI-digested pTN3G11 | 0.29 | 0 |
Values are means ± standard deviations of four independent experiments.
