Figure 6.

Detection sensitivity of Khapra gBlock dsDNA amplicons (upper), evaluating amount of Khapra DNA with synthetic DNA (lower). (a) Amplification profile with templates ranging from 10⁸ to 10 copies at ten‐fold dilution (pink, no amplification). (b) Anneal derivative of LAMP amplicons, with an anneal derivative of ~80 °C. (c) Amplification profile of five‐fold dilution of Khapra DNA (VAITC8332e) ranging from 10 to 1.0⁻⁴ ng μL⁻¹ and synthetic DNA (10⁶ copies, blue and 10⁵ copies, pink). (d) Anneal derivative of LAMP amplicons showing two peaks, ~78 °C for khapra DNA and ~80 °C for synthetic DNA (blue and pink).