Table 2.
DNA extractions and LAMP (loop‐mediated isothermal amplification) assays from intercepted Khapra beetle specimens
| Khapra LAMP | 18S LAMP | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Life stage | Sample | VAITC | Preserved | Extraction type | Extraction method | Time (min) | Anneal derivative (°C) | Time (min) | Anneal derivative (°C) |
| Adult (leg) | AgVic CHS | 8332a | Dry | ‘Clean’ | Destructive column | Failed | Failed | Failed | Failed |
| Adult (leg) | AgVic CHS | 8332c | Dry | ‘Clean’ | Destructive column | 21.5 | 77.5 | 17.2 | 88.7 |
| Larva (whole) | AgVic CHS | 8332d | Ethanol | ‘Clean’ | Destructive column | 16.5 | 77.8 | 13.3 | 89.0 |
| Larva (whole) | AgVic CHS | 8332e | Ethanol | ‘Clean’ | Destructive column | 14.0 | 77.6 | 13.3 | 88.9 |
| Larva (whole) | AgVic CHS | 8332b | Ethanol | ‘Clean’ | Non‐destructive column | 22.0 | 77.5 | 24.2 | 88.1 |
| Larva (whole) | DA 336226 | 9619 | Dry | ‘Clean’ | Non‐destructive column | 20.2 | 77.4 | 13.5 | 88.7 |
| Larva (whole) | DA 336226 | 9620 | Dry | ‘Clean’ | Non‐destructive column | 19.0 | 77.3 | 16.5 | 88.4 |
| Adult (whole) | DA 336226 | 9622 | Dry | ‘Clean’ | Non‐destructive column | 17.2 | 77.5 | 14.0 | 88.6 |
| Larva (whole) | DA 336226 | 9623 | Dry | ‘Clean’ | Non‐destructive column | 22.5 | 77.2 | 18.2 | 88.3 |
| Pupa (whole) | DA 336226 | 9624 | Dry | ‘Clean’ | Non‐destructive column | 23.3 | 77.2 | 24.0 | 88.3 |
| Larva (whole) | DA 336226 | Kh07 | Dry | ‘Crude’ | HS6 | 20.0 | 77.6 | 17.0 | 88.9 |
| Larva (whole) | DA 336226 | Kh08 | Dry | ‘Crude’ | HS6 | 20.0 | 77.8 | 21.5 | 88.8 |
| Larva (whole) | DA 336226 | Kh09 | Dry | ‘Crude’ | HS6 | 23.3 | 77.4 | 17.5 | 88.9 |
| Larva (whole) | DA 335997 | Kh04 | Ethanol | ‘Clean’ | Non‐destructive column | 18.3 | 77.7 | 15.3 | 88.7 |
| Larva (whole) | DA 335997 | Kh05 | Ethanol | ‘Clean’ | Non‐destructive column | Failed | Failed | Failed | Failed |
| Larva (whole) | DA 335997 | Kh06 | Ethanol | ‘Clean’ | Non‐destructive column | 24.2 | 77.3 | 19.0 | 88.8 |
| Larva (whole) | DA 335997 | Kh01 | Ethanol | ‘Crude’ | HS6 | 18.3 | 78.2 | 18.0 | 88.9 |
| Larva (whole) | DA 335997 | Kh02 | Ethanol | ‘Crude’ | HS6 | 16.0 | 78.3 | 15.3 | 88.8 |
| Larva (whole) | DA 335997 | Kh03 | Ethanol | ‘Crude’ | HS6 | Failed | Failed | Failed | Failed |
| Larva (whole) | DA 335997 | Kh10 | Ethanol | ‘Crude’ | HS6 | Failed | Failed | Failed | Failed |
| Larva (whole) | DA 335997 | Kh11 | Ethanol | ‘Crude’ | HS6 | Failed | Failed | Failed | Failed |
| Larva (whole) | DA 335997 | Kh12 | Ethanol | ‘Crude’ | HS6 | Failed | Failed | Failed | Failed |
| Larva (whole) | DA 336226 | Kh13 | Dry | ‘Crude’ | QuickExtract™ | 16.3 | 78.1 | 14.5 | 88.6 |
| Larva (whole) | DA 336226 | Kh14 | Dry | ‘Crude’ | QuickExtract™ | 19.2 | 78.4 | 12.3 | 88.5 |
| Larva (whole) | DA 336226 | Kh15 | Dry | ‘Crude’ | QuickExtract™ | 18.3 | 78.2 | 15.5 | 88.6 |
| Larva (whole) | DA 335997 | Kh16 | Ethanol | ‘Crude’ | QuickExtract™ | 22.5 | 77.9 | 21.3 | 88.7 |
| Larva (whole) | DA 335997 | Kh17 | Ethanol | ‘Crude’ | QuickExtract™ | 22.5 | 77.3 | 21.5 | 88.0 |
| Larva (whole) | DA 335997 | Kh18 | Ethanol | ‘Crude’ | QuickExtract™ | 23.5 | 77.2 | 24.0 | 88.4 |
| Larva (whole) | DA 335997 | Kh19 | Ethanol | ‘Crude’ | QuickExtract™ | 24.2 | 77.1 | 20.3 | 88.7 |
| Larva (whole) | DA 335997 | Kh20 | Ethanol | ‘Crude’ | QuickExtract™ | Failed | Failed | Failed | Failed |
| Larva (whole) | DA 335997 | Kh21 | Ethanol | ‘Crude’ | QuickExtract™ | 24.2 | 77.1 | 17.5 | 88.5 |
| Average: | 20.3 | 77.6 | 17.7 | 88.6 | |||||
| Minimum: | 14.0 | 77.1 | 12.3 | 88.0 | |||||
| Maximum: | 24.2 | 78.4 | 24.2 | 89.0 | |||||
Each line is a different individual insect.