Table 4.
Summary of results for all Khapra beetles tested by various DNA extraction methods, from dry or ethanol preserved samples
| Khapra LAMP | 18S LAMP | |||||||
|---|---|---|---|---|---|---|---|---|
| Time (min) | Temperature (°C) | Time (min) | Temperature (°C) | |||||
| DNA extraction method | Destructive (D), non‐destructive (ND) | Extraction type | Preservation method | n (failed) | mean ± SD | mean ± SD | mean ± SD | mean ± SD |
| Qiagen column | D | ‘Clean’ | Dry | 2 (1) | 21.5 ± 0 | 77.5 ± 0 | 17.2 ± 0 | 88.7 ± 0 |
| Qiagen column | D | ‘Clean’ | Ethanol | 2 (0) | 15.2 ± 1.3 | 77.7 ± 0 | 13.3 ± 0 | 88.9 ± 0 |
| Qiagen column | ND | ‘Clean’ | Dry | 5 (0) | 20.4 ± 2.2 | 77.3 ± 0.1 | 17.3 ± 3.8 | 88.5 ± 0.2 |
| Qiagen column | ND | ‘Clean’ | Ethanol | 4 (1) | 21.5 ± 2.4 | 77.5 ± 0.2 | 19.5 ± 0.3 | 88.5 ± 0.3 |
| HS6 | ND | ‘Crude’ | Dry | 3 (0) | 21.1 ± 1.5 | 77.6 ± 0.2 | 18.6 ± 2.0 | 88.7 ± 0 |
| HS6 | ND | ‘Crude’ | Ethanol | 6 (4) | 17.5 ± 1.1 | 78.3 ± 0 | 16.6 ± 1.3 | 88.8 ± 0 |
| QuickExtract™ | ND | ‘Crude’ | Dry | 3 (0) | 17.9 ± 2.4 | 78.2 ± 0.4 | 14.1 ± 1.3 | 88.6 ± 0 |
| QuickExtract™ | ND | ‘Crude’ | Ethanol | 6 (1) | 23.6 ± 0.8 | 77.2 ± 0.3 | 20.8 ± 2.1 | 88.5 ± 0.2 |
| All methods | 31 (7) | 19.8 ± 2.6 | 77.7 ± 0.4 | 17.2 ± 2.4 | 88.7 ± 0.2 | |||
Individual specimen results are in Table 2. SD, standard deviation.