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. 2021 Nov 18;32(14):3042–3056. doi: 10.1093/cercor/bhab399

Figure 4 .

Figure 4

Shank3b −/− mice lack c-fos mRNA induction in S1 and following whisker stimulation under anesthesia. (A, B) Representative images of c-fos mRNA in situ hybridization in the primary somatosensory cortex (A) and hippocampus (B) of Shank3b+/+ and Shank3b−/− mice, 20 min following anesthesia or whisker stimulation under anesthesia. Scale bars, 500 μm. (C, D) Quantification of c-fos mRNA signal intensity in S1 (C) and hippocampus (D) of Shank3b+/+ and Shank3b−/− mice. Values are expressed as mean normalized signal intensities ±SEM. ****P < 0.0001 Tukey post hoc test following one-way-ANOVA (n = 19 sections from three Shank3b+/+ mice and 18 sections from three Shank3b−/− mice following WS and n = 30 sections from four Shank3b+/+ mice and 32 sections from four Shank3b−/− mice following anesthesia). Genotypes and treatments are as indicated. Abbreviations: CA1/2/3, hippocampal pyramidal cell layers; DG, dentate gyrus; Hp, hippocampus; L2–6, S1 cortical layers; S1, primary somatosensory cortex.