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Validation of 3PM in juvenile SVZ imaging. (A) Confocal imaging of a representative juvenile brain section labeled with CTO (red) and DAPI (blue) at 3 dpi. (B) 3D reconstruction of white matter (THG) and CTO-labeled SVZ from the depth of 1050–1400 μm in a juvenile mouse (3 dpi; red, CTO fluorescence; green, THG) (see Supplementary Movie 2). (C, D) Selected XY frames at different depths in (B). Scale bars represent 1000 μm in A, 100 μm in B, 30 μm in C and D.
