FIGURE 4.

TAN alleviated LPS‐induced cell viability decrease and inflammatory response by promoting VLDLR expression. (a) Western blot was used to detect the transfection efficiency of siVLDLR. (b) Gray value analysis of Western blot results. (c) qRT‐PCR was used to detect the transfection efficiency of siVLDLR. (d) MTT was used to detect the cell viability of FHC cells treated with or without siVLDLR, LPS and TAN. (e–g) ELISA was used to measure the content of IL‐6, IL‐8, IL‐1β in FHC cells treated with or without siVLDLR, LPS and TAN. (h–j) qRT‐PCR was used to identify the expressions of IL‐6, IL‐8, IL‐1β in FHC cells treated with or without siVLDLR, LPS and TAN. GAPDH severs as an internal reference. ^& Vs. siNC, ^* vs. control, ^# vs. LPS + vector, ^ vs. LPS + TAN + vector; ^&& P < .01, ^*** P < .001, ^### P < .001, ^{^^^} P < .001. qRT‐PCR, quantitative reverse transcription polymerase chain reaction; MTT, 3‐(4,5‐Dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide; TAN, tanshinol; IL, interleukin; VLDLR, low density lipoprotein receptor; ELISA, enzyme‐linked immunosorbent assays; FHC, normal colorectal mucosal cell line