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. 2022 Jul 4;9:921160. doi: 10.3389/fvets.2022.921160

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Copyright © 2022 Blake, Jensen, Mabbott, Hope and Stevens.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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RT-qPCR of mRNA expression indicative of specific cell types in enteroid-derived models. The gene expression was determined by RT-qPCR and calculated as fold change relative to the expression of ACTB and REPS1 as endogenous reference genes. Total RNA was isolated from the samples and confirmed to be free of genomic DNA. The results are from samples derived from 2 independent animals, with 3 technical repeats and presented as mean values ± standard deviation (SD). Statistical analysis was performed with a one-way ANOVA; * = P ≤ 0.05; ** = P ≤ 0.01; *** = P ≤ 0.001; **** = P ≤ 0.0001.